Measurement
Part:BBa_K911003:Design
Designed by: Oliver Meacock Group: iGEM12_Cambridge (2012-09-12)
Fluoride Sensitive Riboswitch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 26
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ was used as our reporter. We have included the lysine promoter as part of this biobrick as a reliably constitutively active promoter, ensuring that the rate of transcription of downstream genes should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.
Source
- Bacillus Cereus Genome
- Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a knockout strain of bacillus subtilis.
References
- Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.